Email:export@chinahuida.cn Jiangsu Huida Medical Instruments Co.,Ltd

Jiangsu Huida Medical Instruments Co.,Ltd

The Rapid Detection Method Of Transfer Pipette Accuracy Is Explained

Transfer Pipettes are one of the most commonly used instruments in the laboratory and one of the instruments most likely to affect the results of the experiments, ensuring that the accuracy is very necessary. So how do you ensure the accuracy of the Transfer Pipette during the use of the Transfer Pipette?

First, we have to determine if there is a leak in the Transfer Pipette.

1. Detection method:

1) Visual inspection: first use the need to detect the Transfer Pipette to absorb the liquid, and then put it vertically for 15 seconds to observe whether there is no slow outflow of droplets. If there is a slow outflow of droplets, it shows that the Transfer Pipette has a leak phenomenon.

2) pressure pump detection: the use of a dedicated pressure pump, the pressure test to determine whether the Transfer Pipette leak.

2. If you find the Transfer Pipette leak phenomenon, it is necessary to analyze the possible causes of leakage:

1) Check whether the tip is matched; it is recommended to use the original matching tip.

2) When assembling the tip is not tightened; due to the installation of the tip when there is no tightening caused by Transfer Pipette leakage.

3) If the above two cases are not found in the problem, it is likely that the Transfer Pipette internal air tightness is not good.

When the exclusion of the first case above, we have to troubleshoot the second case:

We need to detect whether the temperature of the liquid is removed and the temperature difference between the Transfer Pipette and the tip causes the accuracy of the Transfer Pipette. In general, the following differences occur when the temperature of the removed liquid does not coincide with the temperature of the tip and the Transfer Pipette:

1. When the liquid temperature is higher than the tip, the volume of liquid removed will be too large;

2. When the liquid temperature is lower than the tip, the volume of liquid removed will be too small.

If the second case is excluded, then we have to troubleshoot the third case below:

Observe the density of liquid samples and whether there is a very significant difference between water. In general, the Transfer Pipette is calibrated at the factory with water as a standard solution, so if the ratio of liquid to water is significantly different, the accuracy and accuracy given may not be satisfied Resulting in an error.

After removing all of the above problems, we should also pay attention to the suction rate should not be too fast, too fast absorption will produce recoil and bubbles, resulting in fluid volume is not accurate, and ultimately affect the accuracy of the Transfer Pipette.


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