Email:export@chinahuida.cn Jiangsu Huida Medical Instruments Co.,Ltd

Jiangsu Huida Medical Instruments Co.,Ltd

Transfer Pipette Use Method Precautions And Routine Maintenance Methods

The specific operation of the pipette: (presumably for you will be useful!) With the thumb and index finger rotation of the upper part of the knob, so that the number of digital window required volume of the volume, in the bottom of the receiver plug a plastic tip And tighten to ensure that the airtight, and then close the four fingers to hold the upper part of the receiver, with the thumb to hold the top of the plunger rod button, press down to the first stop, the suction device into the nozzle Of the solution, slowly release the button, suck the liquid, and stay 1 to 2 seconds.

Use of pipettes

1. Set the volume of the fluid: from a large range of adjustment to a small range for the normal adjustment method, counterclockwise rotation can scale;

When adjusting from a small range to a large range, it should be adjusted to exceed the set volume scale, and then transferred back to the set volume, which can ensure the accuracy of the pipette.

2. Assembly pipette tip: the pipette vertical plug into the tip, left and right rotation half circle, you can tighten.

(Note: the method of using the pipette to hit the tip is very undesirable. Long-term operation back to the part of the pipette is loosened by the impact and will cause the knob to adjust the scale.

3. Suction and liquid:

Vertical suction:

The tip of the tip is immersed in the liquid level below 3mm. Before the liquid, the tip of the liquid is pre-rinsed twice in the liquid to ensure the accuracy and accuracy of the pipetting (because the inner wall of the tip will leave a layer of "liquid film" The amount of small and produce errors);

Slow and slow to take place, to prevent the sudden release of the solution inhalation too fast and into the infusion of the plunger caused by leakage;

If the amount of liquid is very small, it should be a sharp tip of the container.

(When using, check whether there is a leak in the liquid.) Take the liquid and place it vertically for a few seconds to see if the liquid level drops. If the fluid leaks, check whether the suction nozzle matches and the spring piston is normal.

4. Concentration and viscosity of the liquid: will produce errors, in order to eliminate the error compensation, can be determined by the test, the amount of compensation can be used to adjust the reading knob to read the reading window to set. (Reverse transfer technology can be used to remove high viscosity liquid)

5. Liquid-laden pipettes should not be flat: the liquid inside the tip can easily pollute the inside of the gun and may cause the spring of the gun to rust.

6. The pipette should be adjusted to a maximum of the spring return to the prototype after each experiment to extend the service life of the pipette.

7. Pipetting correction:

The weight of the pure water can be weighed by the analytical balance and calculated to correct the extractor, and 1 mL of distilled water weighs 0.9982 g at 20 ° C.

8. Pipettes are strictly prohibited to absorb a strong volatile, strong corrosive liquids (such as concentrated acid, concentrated alkali, organic matter, etc.).

9. Do not use pipettes to blow mix liquid.

10. Do not use a large range of pipettes to remove small volumes of liquid, so as not to affect the accuracy;

Also, if you need to remove a large amount of liquid outside the range, use pipettes to operate.

11. If the liquid is not careful to enter the piston chamber should be timely removal of pollutants;

Regularly clean the outer wall of the pipette, you can use 95% alcohol or 60% isopropyl alcohol, and then wipe with distilled water, naturally dry.

Pipette use precautions

(1) to draw the liquid must be slowly and slowly release the thumb, never allow a sudden release, to prevent the solution inhaled too fast and into the liquid within the plunger caused by leakage.

(2) In order to obtain a higher precision, the suction nozzle must be pre-drawn a sample solution, and then the official pipetting, because the absorption of serum protein solution or organic solvent, the tip of the inner wall will be a layer of "liquid film" The amount of small and produce errors.

(3) the concentration and viscosity of the liquid, will produce errors, in order to eliminate the error compensation, can be determined by the test, the amount of compensation can be used to adjust the reading knob to read the reading window to set.

(4) can be used to calculate the balance of the weight of pure water and calculated by the method to correct the extractor, 1mL distilled water at 20 ℃ when 0.9982g.

(5) the pipette repeatedly hit the tip to tighten the method is very undesirable, long-term operation will make the internal parts loose and damage the pipette.

(6) When the pipette is not filled with the tip, it must be pipetted.

(7) Please pay attention when setting the range. Rotate to the desired range? The numbers are clear in the display window? Set the range in the range of the pipette should not be the knob out of the range, otherwise it will jam the mechanical device, damage the pipette.

(8) pipettes are strictly prohibited to absorb a strong volatile, strong corrosive liquids (such as concentrated acid, concentrated alkali, organic matter, etc.).

(9) It is forbidden to use a pipette to mix the liquid.

(10) Do not use a large range of pipettes to remove small volumes of liquid, so as not to affect the accuracy. Also, if you need to remove a large amount of liquid outside the range, use pipettes to operate.


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